Department of Biochemistry, University of Delhi South Campus

For Ph.D.

 

Ph.D. (Biochemistry) BIOCHEM P-I : RESEARCH METHODOLOGY

Chemical Safety Guidelines for Laboratories
Guidelines for Safety in Biotechnology
Guidelines for Animal Facility
Guidelines_SOP_CPCSEA
Guidelines_ethical_human_ICMR
Guidelines_Radiation
Guidelines_90 RDT Guidelines
Guidelines_Material Safety Data Sheet
WHO Biosafety Manual
Presentations Know How
How to Structure Papers for Publication




Ph.D. (Biochemistry) BIOCHEM P-II : TOOLS AND TECHNIQUES IN BIOCHEMISTRY

Unit 2 : Physical Biochemistry by David Sheehan

Physical Biochemistry David Sheehan

Unit 4

1.A)  Introduction to cell culture
        https://www.thermofisher.com/in/en/home/references/gibco-cell-culture-basics/introduction-to-cell-culture.html 

B)     Animal Cell culture Guide

        https://www.atcc.org/~/media/PDFs/Culture%20Guides/AnimCellCulture_Guide.ashx 

2.     Generation of Stable Expression Mammalian Cell Lines
        https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261361/ 

3.     https://www.addgene.org/protocols/generating-stable-cell-lines/ 

4.     Selecetable markers : https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/selectable-marker 

5.     Transfection and selection in mammalian cells

        https://blog.addgene.org/plasmids-101-mammalian-vectors 

6.      Inducible expression :

         https://www.genoway.com/technologies/tet/tet-system.htm 

         https://www.addgene.org/collections/tetracycline/ 

         https://blog.addgene.org/plasmids-101-inducible-promoters 

 

Unit 5:

Isolation of Microtubules and Microtubule Proteins
Preparation and Coculture of Neurons and Glial Cells
Isolation of Lipid Droplets from cells by Density Gradient Centrifugation
Isolation of Intestinal Brush-Border Membranes
Isolation of Endoplasmic Reticulum, Mito and Mito associated Mem
Isolation of CCV by Differential and Density Gradient Centrifugation
Cell Fraction

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